Sam Burgess has been ruled to Papua New Guinea and New Zealand.
Last 21, the 30-year-old suffered a recurrence of a shoulder injury in the NRL semi-final defeat of his club to Canberra Raiders and can be set to undergo operation.
As they prepare to set out on the Lions tour for 23 decades, the reduction of Burgess, that left his Great Britain debut in 2007 within an 18-year-old from the Kiwis, is a major setback for Wayne Bennetts staff.
Rugby Football League rugby director Kevin Sinfield said on Tuesday:We got it confirmed this morning that Sam Burgess will be from the excursion unfortunately.
It is very disappointing. Hes had an ongoing issue with his shoulder which will likely require surgery. As are we all he is absolutely gutted.
Weve noticed how special it is to all our players, Sam having been among the chosen few previously who have worn the shirt and did outstandingly well inside.
He is a leader and a genuine big role in that dressing room and in the field hes world class so we will surely miss him.
It will offer somebody else having an chance and they can catch it.
The RFL announced a 16-man England squad to the World Cup Nines.
That squad will be boosted by additional players and agents from Saturdays Grand Final between Salford and St Helens before it is announced on Monday.
One of the forward on stand-by is Castleford prop Liam Watts, but Sinfield said:There is still a good deal to be determined.
You would expect a massive contingent to be involved in the Grand Final in the weekend and well piece everything together on Sunday morning.
Weve a great idea of where were in and Wayne is completely across all. Weve had six selectors hard at work pulling squads together, its probably the most stringent selection process weve ever had.
Sinfield confirmed that former Leeds centre Kallum Watkins, now with Gold Coast Titans, was not chosen because of a rib injury and a last decision has yet to be made on the fitness of Wigan loose forward Sean OLoughlin, whos expected to be named Lions captain.
Meanwhile, Sinfield says following demonstrating his eligibility, newly-crowned Man of Steel Jackson Hastings is.
The Salford half-back was born in Australia but, like Warrington stand-off Blake Austin, has an grandparent that was English and he awakened papers to prove his qualification.
We would have liked to own it confirmed a tiny bit earlier, especially once you think how quick Blake was, Sinfield stated.
But we know where Jackson was at and today hes in contention like everyone else.
Its a shame he has missed some of the sessions we conducted through the year but, from my experience of spending a bit of time together with him, hes a quite a sociable lad wholl fit into many dressing rooms